Absorption Spectra of Major Cromophores Used in Photoacoustics
Methylene Blue Spectrum
Methylene blue spectrum was taken by Scott Prahl with a HP spectrophotometer using a 1 cm quartz cuvette filled with 10µM solution of Methylene Blue in water. To convert this data to absorbance A, multiply by the molar concentration and the pathlength.
To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303.
Indocyanine Green Spectrum
Indocyanine green spectrum was taken by Andrew Barofsky with a HP spectrophotometer. The low concentration is very noisy because the data was scanned too quickly for such a concentration.
To convert this data to absorbance A, multiply by the molar concentration and the pathlength.
To convert this data to absorption coefficient in (cm-1), multiply by the molar concentration and 2.303
Simulate Oxy- and Deoxy-Hemoglobin
Solutions Of Cupric and Nickel Sulfate can be used to simulate oxy- and deoxy-hemoglobin. They are very stable and purely aborbing
Absorption Spectra of Biological Components
Wavelength Range of 400 to 2500nm
Blood absorption coefficient, scattering coefficient as well as anisotrophy factor g for the wavelength range of 400 to 2500nm according to
A. Roggan, M. Friebel, K. Dorschel, A. Hahn, and G. Muller, “Optical properties of circulating human blood in the wavelength range 400-2500 NM,” Journal of Biomedical Optics 4, 36-46 (1999).
Mammalian Fat Spectrum
Mammalian Fat spectrum was taken by R.L.P. van Veen and H.J.C.M. Sterenborg, A. Pifferi, A. Torricelli and R. Cubeddu Determination of VIS- NIR absorption coefficients of mammalian fat, with time- #and spatially resolved diffuse reflectance and transmission spectroscopy #OSA Annual BIOMED Topical Meeting, 2004.
Bilirubin retrieved from University of Arizona Database.
Haemoglobin and Oxyhaemoglobin
Haemoglobin and Oxyhaemoglobin retrieved from University of Arizona Database.
Acoustic Properties of Materials
Speed of Sound vs Temperature
Speed of sound vs Temperature compiled by Peter Brecht. Range of validity: 0-95°C at atmospheric pressure. according to formula by
Marczak (1997) combined three sets of experimental measurements, Del Grosso and Mader (1972), Kroebel and Mahrt (1976) and Fujii and Masui (1993) and produced a fifth order polynomial based on the 1990 International Temperature Scale.
More Acoustic Properties
Additional Spectra Database
The University of Arizona is hosting a Spectra Database of Dyes (Absorprion/Emmission), Filters, Dichroic Mirrors and Light Sources. This link will take you to the Spectraviewer. All spectras are downloadable in CSV format.